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Summary of our research
Research group, including Dr. Keiki Nagaharu (Graduate student, Mie University Graduate School of Medicne), Dr. Kohshi Ohishi (Director & Associate Professor, Transfusion Medicine and Cell Therapy, Mie University Hospital), Dr. Yasuhiro Kojima (Specially Appointed Lecturer, Division of Systems Biology, Nagoya University Graduate School of Medicine), Dr. Teppei Shimamura (Professor, Division of Systems Biology, Nagoya University Graduate School of Medicine), and Dr. Hiroyoshi Nishikawa (Professor, Department of Immunology, Nagoya University Graduate School of Medicine & Division of Cancer Immunology, Research Institute, National Cancer Center), discovered that B cells (which produce antibody) and plasmacytoid denderitic cells (pDCs)(which produce type 1 interferon in response to a viral infection) originate from common progenitors with strong fluctuation of trasncriptome dynamics and that LFA-1 fluctuates positively in the pDC direction at the bifurcation, using an original computational methodology and an advanced lymphoid coculture system. Furthremore, single cell multiome analysis revealed that the differentially accessible chromatin regions of B cells and pDCs were simultaneously accessible in the cell population with a large fluctuation of transcriptome dynamics by suport of "Platform for Advanced Genome Science" (PAGS). Hematopoiesis was considered a hierarchical stepwise process, but revised to a continuous process by single cell RNA-sequencing. We provide a new model of differentiation, fluctuation-based differentiation, which reconciles continuous and discrete models.
Researcher information
NAGAHARU Keiki
Graduate student (present Research Associate), Department of Hematology and Oncology, Mie University Graduate School of Medicine (present Yokkaichi Municipal Hospital)
Specialized area:
Hematology
Current research field:
Eluciadation of molecular mechanism and fibrosis of myeloproliferative neaoplasms
OHISHI Kohshi
Director, Associate Professor, Transfusion Medicine and Cell Therapy, Mie University Hospital
Specialized area:
Hematology, trasnfusion, HIV infection
Current research field:
Elucidation of human lymphoid pathway and novel differentiation mechanism